Three-year outcomes after genotyping-guided treatment switch in HIV patients with virologic failure in Cameroon: Implications for epidemic control in low- and middle-income countries

Ezechiel Ngoufack Jagni Semengue at all

Abstract

Objectives: Monitoring of HIV-infected patients with long-term treatment remains challenging in low- and middle-income countries (LMICs) due to risks of multi-class HIV drug resistance (HIVDR). We sought to evaluate the treatment response after a genotypic resistance-guided treatment switch among patients with multi-class HIVDR in Cameroon. Methods: A retrospective cohort study was conducted in the South-West region of Cameroon among pa- tients failing first- and second-line antiretroviral treatment (ART) (i.e., non-nucleoside reverse transcrip- tase inhibitors (NNRTI)- and protease inhibitor (PI)-based) from 2018 to 2023. Following HIV-1 genotypic resistance testing (GRT), patients were switched to the most effective genotype-guided therapies and vi- ral load (VL) was monitored thereafter. Data analysis was performed using Epi-Info v.7.2, with P < 0.05 considered significant. Results: Overall, 81 patients were included in the study (median CD4 and VL were 232.5 [161.25–401.25] cells/μL and 54,480 [13,932.5–220,153] copies/mL, respectively). HIVDR at failure (i.e., VL > 10 0 0copies/mL) was 61.7%, and prevailing mutations were M184V (24.6%), K103N (17%) and M46I (32.1%) for NRTI, NNRTI and PI respectively. Monitoring data revealed 92.6% of adherence to the prescribed regimen. Viral sup- pression post-GRT was 80% at 3-months, 84.8% at 6-months, 81.4% at 12-months, 93.7% at 24-months